概述

  • 产品名称小鼠Axl ELISA试剂盒
    参阅全部 Axl 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 1 pg/ml
  • 范围
    1 pg/ml - 250 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 93.26 81% - 105%
    Serum 95.29 82% - 104%
    Plasma 97.56 84% - 106%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam's Axl Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Axl in serum, plasma and cell culture supernates. 

    This assay employs an antibody specific for mouse Axl coated on a 96-well plate. Standards and samples are pipetted into the wells and Axl present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Axl antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Axl bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2).
  • 别名
    • Adhesion related kinase
    • AI323647
    • Ark
    • AXL oncogene
    • AXL receptor tyrosine kinase
    • AXL transforming gene
    • AXL transforming sequence/gene
    • JTK11
    • Oncogene AXL
    • Tyro7
    • Tyrosine protein kinase receptor UFO
    • UFO
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Axl ELISA Kit 图像

  • Representative Standard Curve using ab100669.

  • Representative Standard Curve using ab100669.

实验方案

Mouse Axl ELISA Kit (ab100669)参考文献

ab100669 has not yet been referenced specifically in any publications.

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