小鼠Antithrombin III ELISA试剂盒(SERPINC1) (ab108800)

概述

  • 产品名称小鼠Antithrombin III ELISA试剂盒(SERPINC1)
    参阅全部 Antithrombin III 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.4%
    批次间
    样品 n Mean SD CV%
    Overall 9.4%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 3 ng/ml
  • 范围
    3.13 ng/ml - 100 ng/ml
  • 复检

    96 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.

    An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • 组织特异性Found in plasma.
  • 疾病相关Antithrombin III deficiency
  • 序列相似性Belongs to the serpin family.
  • 翻译后修饰Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 别名
    • ANT3_HUMAN
    • Antithrombin-III
    • ATIII
    • Serpin C1
    • SERPINC1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Antithrombin III ELISA Kit (SERPINC1) 图像

  • Standard curve : mean of duplicates (+/-SD) with backgrounds readings subtracted

  • Ms antithrombine III measured in biological fluids showing quantity (ng) per mL of sample tested

实验方案

Mouse Antithrombin III ELISA Kit (SERPINC1) (ab108800)参考文献

ab108800 has not yet been referenced specifically in any publications.

Product Wall

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The standards for these kits are supplied in different masses. The mass for each vial will be printed on the label, and each lot will require a different volume of diluent in order to generate the 100 ng/mL standard....

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Yes, I can confirm that the vial you received is 50x which is correct in this case - for the lot you received. We are looking into updating the datasheet. Going forward 80x will be the correct amount.

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Thank you for contacting us. I have had a chance to speak with the lab regarding your question. Our data suggested that mouse serum ATIII has slightly higher concentration than plasma. We have about 200 – 600 ug/ml range of different mouse samples....

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Thank you for contacting us. I was hoping that you might fill me in on a couple of points about your results with this assay. Have you been able to plot the standard curve accurately using the mean value of the triplicate readings for each stan...

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