小鼠Antithrombin III ELISA试剂盒(SERPINC1) (ab108800)
Key features and details
- Sensitivity: 1.7 ng/ml
- Range: 6.25 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠Antithrombin III ELISA试剂盒(SERPINC1)
参阅全部 Antithrombin III/ATIII 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6.2% 批次间 样品 n Mean SD CV% Overall 10.7% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 1.7 ng/ml -
范围
6.25 ng/ml - 50 ng/ml -
回收率
96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.
An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse Antithrombin III Antibody 1 x 120µl Antithrombin III Standard 1 vial Antithrombin III Microplate (12 x 8 well strips) 1 unit Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
组织特异性
Found in plasma. -
疾病相关
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
序列相似性
Belongs to the serpin family. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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别名
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
数据库链接
- Entrez Gene: 11905 Mouse
- SwissProt: P32261 Mouse
- Unigene: 260770 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108800 被引用在 1 文献中.
- Foster DJ et al. Advanced siRNA Designs Further Improve In Vivo Performance of GalNAc-siRNA Conjugates. Mol Ther 26:708-717 (2018). PubMed: 29456020