小鼠and兔Specific HRP Plus Detection IHC试剂盒(Ready To Use) (ab93697)
概述
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产品名称
小鼠and兔Specific HRP Plus Detection IHC试剂盒(Ready To Use)
参阅全部 Polyvalent HRP 试剂盒 -
产品概述
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system with increased sensitivity. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
ab93697 does not contain chromogen or substrate.
Reacts with Mouse and Rabbit IgG (H+L).
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经测试应用
适用于: IHC-Pmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 125 ml 60 ml Biotinylated Goat Anti-Polyvalent Plus 1 x 125ml 1 x 60ml Protein block 1 x 125ml 1 x 60ml Streptavidin Peroxidase Plus 1 x 125ml 1 x 60ml -
研究领域
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相关性
Polyvalent HRP is a labeled streptavidin-biotin immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. -
别名
- Polyvalent/HRP
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent dilution.
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说明 |
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IHC-P
Use at an assay dependent dilution. |
图片
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Immunohistochemistry analysis of mouse femur tissue sections. Femurs were fixed in 10% neutral buffered formalin and decalcified for 7 days in 15% EDTA; complete decalcification was confirmed by X-ray. Decalcified bones were embedded in paraffin and sectioned longitudinally through the defect at a thickness of 8 microns. Immunohistochemical staining was performed with monoclonal anti-activated beta-catenin (un-phosphorylated on Ser33/37 and Thr41), or an IgG isotype control. Chromogens were developed using ab93697, Mouse and Rabbit Specific HRP Plus Detection IHC Kit (Ready to Use), followed by 3′-3′-diaminobenzidine (DAB), and then counterstained with fast green. Beta-catenin levels are elevated in defects of Sost-/- mice.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab93697 被引用在 23 文献中.
- Rahimian G et al. Relationship between mucosal TNF-α expression and Th1, Th17, Th22 and Treg responses in Helicobacter pylori infection. AMB Express 12:113 (2022). PubMed: 36057049
- Giglia G et al. West Nile Virus and Usutu Virus: A Post-Mortem Monitoring Study in Wild Birds from Rescue Centers, Central Italy. Viruses 14:N/A (2022). PubMed: 36146800
- Weaver SR et al. Pleckstrin homology (PH) domain and Leucine Rich Repeat Phosphatase 1 (Phlpp1) Suppresses Parathyroid Hormone Receptor 1 (Pth1r) Expression and Signaling During Bone Growth. J Bone Miner Res 36:986-999 (2021). PubMed: 33434347
- Fattahi S et al. Correlation between aryl hydrocarbon receptor and IL-17+ and Foxp3+ T-cell infiltration in bladder cancer. Int J Exp Pathol 102:249-259 (2021). PubMed: 34762773
- Azadegan-Dehkordi F et al. Increased Indoleamine 2, 3-Dioxygenase expression modulates Th1/Th17/Th22 and Treg pathway in humans with Helicobacter Pylori-Infected gastric mucosa. Hum Immunol 82:46-53 (2021). PubMed: 33127161