小鼠alpha Macroglobulin ELISA试剂盒(ab108887)

概述

  • 产品名称
    小鼠alpha Macroglobulin ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.07 ng/ml
  • 范围
    0.078 ng/ml - 20 ng/ml
  • 复检

    96 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s alpha Macroglobulin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Macroglobulin levels in plasma, serum, urine, and cell culture supernatants.

    An alpha Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Macroglobulin captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Typical standard curve using ab108887.

实验方案

文献

ab108887 has not yet been referenced specifically in any publications.

客户评价及客户问答

The locations of the epitopes that the antibodies in the alpha macroglobulin ELISA recognize are unknown. In other words, they have not been mapped.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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