小鼠alpha Macroglobulin ELISA试剂盒(ab108887)
Key features and details
- Sensitivity: 0.28 ng/ml
- Range: 1 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
概述
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产品名称
小鼠alpha Macroglobulin ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.3% 批次间 样品 n Mean SD CV% Overall 10.8% -
样品类型
Cell culture supernatant, Urine, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.28 ng/ml -
范围
1 ng/ml - 25 ng/ml -
回收率
97 %
-
检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse -
产品概述
Abcam’s alpha Macroglobulin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Macroglobulin levels in plasma, serum, urine, and cell culture supernatants.
An alpha Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Macroglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated alpha Macroglobulin Antibody 1 x 120µl alpha Macroglobulin Microplate (12 x 8 well strips) 1 unit alpha Macroglobulin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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数据库链接
- SwissProt: Q61838 Mouse
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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