小鼠Albumin ELISA试剂盒(ab108792)

概述

  • 产品名称小鼠Albumin ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2.5%
    批次间
    样品 n Mean SD CV%
    Overall 9.9%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.7 ng/ml
  • 范围
    1.56 ng/ml - 400 ng/ml
  • 复检

    97 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Albumin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性Plasma.
  • 疾病相关Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Albumin ELISA Kit 图像

  • Representative Standard Curve Using ab108792.

Mouse Albumin ELISA Kit (ab108792)参考文献

This product has been referenced in:
  • Gai Z  et al. Farnesoid X Receptor Protects against Kidney Injury in Uninephrectomized Obese Mice. J Biol Chem 291:2397-411 (2016). Read more (PubMed: 26655953) »
  • Li J  et al. The dipeptidyl peptidase-4 inhibitor sitagliptin protects against dyslipidemia-related kidney injury in Apolipoprotein E knockout mice. Int J Mol Sci 15:11416-34 (2014). ELISA . Read more (PubMed: 24972137) »

See all 5 Publications for this product

Product Wall


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCyto...

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Thank you for your enquiry. I am sorry to hear the customer has some concerns regarding this kit, I hope I can be of help. For the standard,  as described in the protocol this needs to be reconstituted with the appropriate amount of diluent to gene...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"