概述

  • 产品名称
    小鼠Albumin ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2.5%
    批次间
    样品 n Mean SD CV%
    Overall 9.9%
  • 样品类型
    Cell culture supernatant, Urine
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.7 ng/ml
  • 范围
    1.56 ng/ml - 400 ng/ml
  • 复检

    97 %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s Albumin Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Albumin levels in urine and cell culture supernatants.


    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Albumin captured in plate.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve Using ab108792.

实验方案

文献

This product has been referenced in:
  • Chakraborty K  et al. The mito-DAMP cardiolipin blocks IL-10 production causing persistent inflammation during bacterial pneumonia. Nat Commun 8:13944 (2017). Read more (PubMed: 28074841) »
  • Xie Y  et al. Anti-diabetic effect of Alpinia oxyphylla extract on 57BL/KsJ db-/db- mice. Exp Ther Med 13:1321-1328 (2017). Mouse . Read more (PubMed: 28413472) »

See all 9 Publications for this product

客户评价及客户问答


ab108792: We have never tested brain tissue supernatants so I do not know if it would work. I cannot recommend the dilution range.

ab119557: Again, we have not tested brain tissue with this kit but there is a publication where a FlowCyto...

Read More

Thank you for your enquiry. I am sorry to hear the customer has some concerns regarding this kit, I hope I can be of help. For the standard,  as described in the protocol this needs to be reconstituted with the appropriate amount of diluent to gene...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册