小鼠Activin A ELISA试剂盒(ab119569)

概述

  • 产品名称小鼠Activin A ELISA试剂盒
    参阅全部 Activin A 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse
  • 产品概述

    Abcam’s mouse Activin A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse Activin A in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

    A Activin A specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Activin A is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Activin A amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
  • 细胞定位Secreted
  • 别名
    • Activin beta A chain
    • Beta 1
    • erythroid differentiation factor
    • erythroid differentiation protein
    • follicle stimulating hormone releasing protein
    • FSH releasing protein
    • Inhibin
    • inhibin beta A chain
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Mouse Activin A ELISA Kit 图像

  • Representative Standard Curve using ab199569.

实验方案

Mouse Activin A ELISA Kit (ab119569)参考文献

ab119569 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Geduld.

Leider waren wir vom Deutschen Technischen Dienst diese Woche nicht erreichbar, mir wurde aber mitgeteilt, dass Sie allerdings von meiner Kollegin Dr. B. kontaktiert wurden.

Wir benötigen noch ei...

Read More

Vielen Dank für Ihren Anruf den anderen Tag.

Das Labor hat mir bestätigt, dass Sie dieses Kit auf Normalem Maus Serum getestet haben, mit Resultaten zwischen 76-320pg/ml. Der Durchschnittswert war 180pg/ml.

Entspricht dies...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"