抗小鼠IgG for IP (Biotin) (ab131367)
Key features and details
- Anti-mouse IgG for IP (Biotin)
- Conjugation: Biotin
- Host species: Rat
- Isotype: IgG
- Suitable for: WB
概述
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产品名称
抗小鼠IgG for IP (Biotin)
参阅全部 IgG 二抗 -
宿主
Rat -
靶标种属
Mouse -
特异性
VeriBlot for IP secondary antibodies are anti-mouse immunoblotting reagents that enable the trouble-free detection of immunoblotted target protein bands, without interference from denatured IgG. This allows to detect the (co-)immunoprecipitated protein without masking by the IgG heavy (50 kDa) and light chains (25 kDa). In general this interference tends to originate from secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself. VeriBlot for IP secondary antibodies only recognize native (non-reduced) antibodies and therefore the detection of heavy and light chains is highly minimized, if the immunoprecipitate is fully reduced. -
经测试应用
适用于: WBmore details -
偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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克隆
单克隆 -
克隆编号
eB144 -
同种型
IgG -
常规说明
Number of blots:
At least 20 blots (based on a 0.5 µg/ml dilution in 5 ml milk).
Important protocol notes:
1. The anti-mouse VeriBlot for IP secondary antibody (Biotin) detects mouse IgG antibodies (subtypes IgG1, IgG2a, IgG2b, IgG3).
2. The anti-mouse VeriBlot for IP secondary antibody (Biotin) preferentially detects the non-reduced form of mouse IgG (subtypes: IgG1, IgG2a, IgG2b, IgG3) over the reduced, SDS-deantured forms.
3. IP sample should be completely reduced/denatured before loaded onto a western blot.
4. Milk should be used as the blocking protein for the immunoblot.
5. Anti-mouse IgG VeriBlot for IP secondary antibody (Biotin) (ab131367) is the biotinylated form of the VeriBlot for IP anti-mouse HRP (ab131368). It can be used as a secondary antibody in combination with avidin/streptavidin HRP (ab59653/ab7403).
Western blot and IP resources:
a) Western blot a beginner's guide
b) IP protocol
c) IP troubleshooting tips -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131367于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 µg/ml. The dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP.
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说明 |
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WB
Use a concentration of 0.5 µg/ml. The dilution will depend on the sensitivity of the avidin/streptavidin HRP. The recommended is dilution is 0.5 µg/ml. Based on a 0.5 µg/ml dilution in 5 ml milk researchers can perform 20 western blots. This product is recommended for the western blot detection of IP samples in combination with avidin/streptavidin HRP. |
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131367 被引用在 1 文献中.
- Yu Z et al. Translocation of gasdermin D induced mitochondrial injury and mitophagy mediated quality control in lipopolysaccharide related cardiomyocyte injury. Clin Transl Med 12:e1002 (2022). PubMed: 36030524