概述

  • 产品名称Anti-Morg1抗体- Aminoterminal end
    参阅全部 Morg1 一抗
  • 描述
    兔多克隆抗体to Morg1 - Aminoterminal end
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence near the N-terminus of human Morg1, identical to the related mouse and rat sequence.

  • 阳性对照
    • Mammary cancer tissue

性能

应用

Our Abpromise guarantee covers the use of ab65870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.
ICC Use at an assay dependent concentration.

靶标

  • 功能Molecular scaffold protein for various multimeric protein complexes. Acts as a module in the assembly of a multicomponent scaffold for the ERK pathway, linking ERK responses to specific agonists. At low concentrations it enhances ERK activation, whereas high concentrations lead to the inhibition of ERK activation. Also involved in response to hypoxia by acting as a negative regulator of HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote degradation of HIF1A. May act by recruiting signaling complexes to a specific upstream activator (By similarity). May also be involved in pre-mRNA splicing.
  • 序列相似性Belongs to the WD repeat MORG1 family.
    Contains 7 WD repeats.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Partially nuclear.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAPK organizer 1 antibody
    • MGC4238 antibody
    • Mitogen activated protein kinase organizer 1 antibody
    • Mitogen-activated protein kinase organizer 1 antibody
    • MORG 1 antibody
    • WD repeat domain-containing protein 83 antibody
    • wdr83 antibody
    • WDR83_HUMAN antibody
    see all

Anti-Morg1 antibody - Aminoterminal end 图像

  • This image shows human mammary cancer tissue stained with ab65870 at 1µg/ml. Cytoplasmic staining of Morg1, using DAB.
  • Anti-Morg1 antibody - Aminoterminal end (ab65870) at 1 µg/ml + rat brain tissue lysate

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ICC/IF image of ab65870 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65870, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Morg1 antibody - Aminoterminal end (ab65870)参考文献

This product has been referenced in:
  • Su WY  et al. Bidirectional regulation between WDR83 and its natural antisense transcript DHPS in gastric cancer. Cell Res : (2012). ICC/IF . Read more (PubMed: 22491477) »

See 1 Publication for this product

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