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Mouse lymph node stroma
Our Abpromise guarantee covers the use of ab33451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 24449804|
|Flow Cyt||Use at an assay dependent concentration.
Membrane permeabilisation is required for this application.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab33451 staining mouse brain tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 18 hours at 4°C. A HRP conjugated goat anti-rat antibody, diluted 1/500, was used as the secondary.
This antibody labels perivascular (left) and subpial (right) macs in normal brain tissue.