Anti-Monocarboxylic acid transporter 1抗体(ab85021)

概述

  • 产品名称Anti-Monocarboxylic acid transporter 1抗体
    参阅全部 Monocarboxylic acid transporter 1 一抗
  • 描述
    兔多克隆抗体to Monocarboxylic acid transporter 1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Monocarboxylic acid transporter 1.

    (Peptide available as ab97985.)

  • 阳性对照
    • This antibody gave a positive signal in Human Skeletal Muscle tissue lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab85021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
  • 组织特异性Widely expressed in normal and in cancer cells.
  • 疾病相关Symptomatic deficiency in lactate transport
    Familial hyperinsulinemic hypoglycemia 7
  • 序列相似性Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ36745 antibody
    • HHF7 antibody
    • MCT 1 antibody
    • MCT antibody
    • MGC44475 antibody
    • Monocarboxylate transporter 1 antibody
    • Monocarboxylate transporter antibody
    • Monocarboxylate transporter isoform 1 antibody
    • Monocarboxylic acid transporter 1 antibody
    • MOT1_HUMAN antibody
    • Slc16a1 antibody
    • SLC16A1 protein antibody
    • Solute carrier family 16 (monocarboxylic acid transporters) member 1 antibody
    • Solute carrier family 16 member 1 (monocarboxylic acid transporter 1) antibody
    • Solute carrier family 16 member 1 antibody
    see all

Anti-Monocarboxylic acid transporter 1 antibody 图像

  • Anti-Monocarboxylic acid transporter 1 antibody (ab85021) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
    Additional bands at : 40 kDa,89 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab85021 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85021, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
  • IHC image of Monocarboxylic acid transporter 1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85021, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Monocarboxylic acid transporter 1 antibody (ab85021)参考文献

This product has been referenced in:
  • Jensen DH  et al. A reverse Warburg metabolism in oral squamous cell carcinoma is not dependent upon myofibroblasts. J Oral Pathol Med N/A:N/A (2014). IHC-P ; Human . Read more (PubMed: 25420473) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Oral squamous cell carcinoma)
Antigen retrieval step Heat mediated
Permeabilization No
Specification Oral squamous cell carcinoma
Blocking step VENTANA Benchmark ultra as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 00% · Temperature: 37°C
Fixative Paraformaldehyde
Username

David Hebbelstrup Jensen

Verified customer

提交于 Jun 29 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (H1299 Lung cancer cells)
Specification H1299 Lung cancer cells
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Feb 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 minutes
Sample Human Tissue sections (appendix testis)
Specification appendix testis
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 15 2014

Thank you for your reply.

I am very happy to hear that the antibody is now also working well in WB.

We would like to make the improved protocol available to other users and therefore I would like to ask you to write an Abreview for ...

Read More

Thank you for confirming these details and for your cooperation. I contacted our in house QC department and they will have a closer look at ab85021.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience ...

Read More
Application Western blot
Sample Mouse Cell lysate - whole cell (glioma cell, placenta, MDCK, HEK, HL-1)
Loading amount 35 µg
Specification glioma cell, placenta, MDCK, HEK, HL-1
Gel Running Conditions Reduced Denaturing (10)
Blocking step FCS as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mrs. Basler

Verified customer

提交于 Aug 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"