Anti-Monoamine Oxidase B抗体(ab88510)

概述

  • 产品名称
    Anti-Monoamine Oxidase B抗体
    参阅全部 Monoamine Oxidase B 一抗
  • 描述
    小鼠多克隆抗体to Monoamine Oxidase B
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Common marmoset
  • 免疫原

    Full length human protein (NP_000889.3)

  • 阳性对照
    • Human liver lysate. Human kidney tissue. heLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
    • 序列相似性
      Belongs to the flavin monoamine oxidase family.
    • 细胞定位
      Mitochondrion outer membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Adrenalin oxidase antibody
      • Amine oxidase (flavin containing) antibody
      • Amine oxidase [flavin-containing] B antibody
      • AOFB_HUMAN antibody
      • MAO, brain antibody
      • MAO, platelet antibody
      • MAO-B antibody
      • MAOB antibody
      • MGC26382 antibody
      • Monoamine oxidase B antibody
      • Monoamine oxidase type B antibody
      • OTTHUMP00000023166 antibody
      • Tyramine oxidase antibody
      see all

    图片

    • Anti-Monoamine Oxidase B antibody (ab88510) at 1 µg/ml + Human liver lysate at 50 µg

      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • All lanes : Anti-Monoamine Oxidase B antibody (ab88510) at 1 µg/ml

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • Purified ab88510 at 3ug/ml staining Monoamine Oxidase B in formalin-fixed, paraffin embedded Human kidney tissue.
    • Immunofluorescent staining of Monoamine Oxidase B in HeLa cells using purified ab88510 at 10ug/ml.

    文献

    This product has been referenced in:
    • Grouzmann E  et al. Monoamine oxidase a down-regulation contributes to high metanephrine concentration in pheochromocytoma. J Clin Endocrinol Metab 97:2773-81 (2012). Human . Read more (PubMed: 22569243) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Sample
    Marmoset (common) Tissue lysate - whole (brain)
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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