Anti-Monoamine Oxidase B抗体(ab47988)

概述

  • 产品名称Anti-Monoamine Oxidase B抗体
    参阅全部 Monoamine Oxidase B 一抗
  • 描述
    山羊多克隆抗体to Monoamine Oxidase B
  • 特异性This antibody is not expected to cross-react with Monoamine oxidase A.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-HKARKLARLTKEE

    , corresponding to internal sequence amino acids 347-359 of Human Monoamine Oxidase B

  • 阳性对照
    • Human Brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab47988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 21873469

靶标

Anti-Monoamine Oxidase B antibody 图像

  • All lanes : Anti-Monoamine Oxidase B antibody (ab47988) at 1 µg/ml

    Lane 1 : Human Brain lysate (35µg protein in RIPA buffer) with no blocking with the immunizing peptide
    Lane 2 : Human Brain lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide


    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-Monoamine Oxidase B antibody (ab47988) at 0.5 µg/ml + Human brain (hippocampus) lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

Anti-Monoamine Oxidase B antibody (ab47988)参考文献

This product has been referenced in:
  • Francis H  et al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut 61:753-64 (2012). ICC/IF ; Human . Read more (PubMed: 21873469) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. The protocol is as follows: ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"