The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 59 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
序列相似性Belongs to the flavin monoamine oxidase family.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B antibody (ab118865)
ab118865 at 5 µg/ml staining Monoamine Oxidase B in Paraffin-embedded Human brain cortex tissue by immunohistochemistry. After incubation with ab118865 slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-Monoamine Oxidase B antibody (ab118865)
All lanes : Anti-Monoamine Oxidase B antibody (ab118865) at 1 µg/ml
Lane 1 : Human brain lysate without blocking peptide Lane 2 : Human brain lysate with blocking peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique
Predicted band size : 59 kDa
Anti-Monoamine Oxidase B antibody (ab118865)参考文献
has not yet been referenced specifically in any publications.