Anti-Monoacylglycerol Lipase抗体(ab24701)

概述

  • 产品名称
    Anti-Monoacylglycerol Lipase抗体
    参阅全部 Monoacylglycerol Lipase 一抗
  • 描述
    兔多克隆抗体to Monoacylglycerol Lipase
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Monkey, African green monkey
  • 免疫原

    Recombinant fragment:

    MPEESSPRRTPQSI

    , corresponding to amino acids 1-14 of Human Monoacylglycerol Lipase

  • 阳性对照
    • IHC: white adipose tissue (visceral & mesenteric adipose tissue), brown adipose tissue, colon and testis. WB: liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab24701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
WB 1/200. Predicted molecular weight: 33 kDa.Can be blocked with Monoacylglycerol Lipase peptide (ab82022).

靶标

  • 功能
    Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
  • 组织特异性
    Detected in adipose tissue, lung, liver, kidney, brain and heart.
  • 通路
    Glycerolipid metabolism; triacylglycerol degradation.
  • 序列相似性
    Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 3.1.1.23 antibody
    • HU K5 antibody
    • HU-K5 antibody
    • HUK5 antibody
    • Lysophospholipase homolog antibody
    • Lysophospholipase like antibody
    • Lysophospholipase-like antibody
    • MAGL antibody
    • MGL antibody
    • MGLL antibody
    • MGLL_HUMAN antibody
    • Monoacylglycerol lipase antibody
    • Monoglyceride lipase antibody
    see all

Anti-Monoacylglycerol Lipase antibody 图像

  • Anti-Monoacylglycerol Lipase antibody (ab24701) at 1/200 dilution + Rat brown fat at 20 µg

    Predicted band size : 33 kDa
  • ab24701 in 1/200 dilution staining Monoacylglycerol Lipase in mouse visceral adipose tissue: The large "holes" indicate the presence of the white adipocytes as they accumulate fat stores in one lipid droplet that fills the whole cell thus pushing the cytoplasm to the periphery only, whereas brown adipocytes accumulate cytoplasmic lipid in multiple droplets. The tissue to the right is the hilus of mouse kidney and is substantially negative for this Ab reagent.

    See Abreview

  • ab24701 staining Monoacylglycerol Lipase from monkey mesenteric adipose tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 1% BSA for 10 minutes at RT. The primary antibody was diluted 1/200 and incubated with the sample for 2 hours. A biotin goat polyclonal to rabbit IgG, diluted 1/200, was used as the secondary.

    See Abreview

Anti-Monoacylglycerol Lipase antibody (ab24701)参考文献

This product has been referenced in:
  • Sokolina K  et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol 13:918 (2017). IP . Read more (PubMed: 28298427) »
  • Woo J & Kang S Diet change and exercise enhance protein expression of CREB, CRTC 2 and lipolitic enzymes in adipocytes of obese mice. Lipids Health Dis 15:147 (2016). Mouse . Read more (PubMed: 27596982) »

See all 8 Publications for this product

Product Wall

Thank you for contacting Abcam.

The blocking peptide ab82022, is the correct one to use in conjunction with ab24701. They both have the same immunogen sequence.

If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (olfactory epithelium)
Loading amount
40 µg
Specification
olfactory epithelium
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12.5%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Mesenteric adipose tissue/colon)
Specification
Mesenteric adipose tissue/colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Oct 20 2009

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neural Stem Cell)
Specification
Neural Stem Cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Madeleine Oudin

Verified customer

提交于 Jun 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Visceral adipose tissue)
Specification
Visceral adipose tissue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid ph6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brown/White fat and testis)
Specification
Brown/White fat and testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 30 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (hepatocyte)
Loading amount
40 µg
Specification
hepatocyte
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 30 2008

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (hepatocytes)
Specification
hepatocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 28 2008

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hepatocytes)
Specification
hepatocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 28 2008

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 28 2008

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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