Monkey Soluble TNF Receptor I ELISA试剂盒(ab119562)

概述

  • 产品名称
    Monkey Soluble TNF Receptor I ELISA试剂盒
    参阅全部 TNF Receptor I 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4 2%
    批次间
    样品 n Mean SD CV%
    Overall 4 9%
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.06 U/ml
  • 范围
    0.09 U/ml - 6 U/ml
  • 复检

    93 %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s Soluble TNF Receptor I Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey Soluble TNF Receptor I concentrations in Cell culture supernatant and serum (baboon, cynomolgus).

    Soluble TNF Receptor I specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated Soluble TNF Receptor I detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is then added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the Soluble TNF Receptor I amount of sample captured in plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119562.

实验方案

文献

ab119562 has not yet been referenced specifically in any publications.

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