Monkey IL-8 ELISA试剂盒(Interleukin-8) (ab119550)


  • 产品名称Monkey IL-8 ELISA试剂盒(Interleukin-8)
    参阅全部 IL8 试剂盒
  • 检测方法Colorimetric
  • 精确度
    样品 n Mean SD CV%
    Overall 6.3%
    样品 n Mean SD CV%
    Overall 8.7%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    3 U/ml
  • 范围
    19.5 U/ml - 1250 U/ml
  • 复检

    88 %

    样品类型 平均% 范围
    Serum 88 72% - 125%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s IL-8 (Interleukin-8) Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of monkey IL-8 in culture supernatant and serum (cynomolgus, baboon).

    IL-8 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a biotin-conjugated anti-monkey IL-8 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured on the plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate


  • 功能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • 3-10C
    • AMCF-I
    • B-ENAP
    • Beta-thromboglobulin-like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Interleukin 8
    • K60
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG-1
    see all


Our Abpromise guarantee covers the use of ab119550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Monkey IL-8 ELISA Kit (Interleukin-8) 图像

  • Representative Standard Curve using ab119550.


Monkey IL-8 ELISA Kit (Interleukin-8) (ab119550)参考文献

ab119550 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
The unit in the monkey kit is always the same as in the human kits. In this case it is pg/ml. Thus, 125 Units/ml is around 125 pg/ml.
I hope this information is helpful to you. Please do not hesitate to contact us...

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Thank you for your reply. My colleague is out of the office today but I am happy to help you with this in her absence.
I've asked the lab about the standard in this kit, and it is actually a recombinant human protein.
I hope this information ...

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Thank you for contacting us.
Yes, ab119550 has been validated for use on baboon and cynomolgus samples. I hope this helps, please let me know if you need any additional information or assistance.