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Abcam’s IL-12 p70 (Interleukin-12 p70) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-12 p70 concentrations in Cell culture supernatant and serum (baboon).
Monkey IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Monkey IL-12 p70 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Monkey IL-12 p70 captured on the plate.
Standard is recombinant protein produced in E.coli.
|组件||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-monkey IL12 p70 monoclonal antibody||1 x 100µl|
|Microplate coated with monoclonal antibody to monkey IL-12 p70 (12 x 8 wells)||1 unit|
|Monkey IL12 p70 Standard||2 vials|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119533 has not yet been referenced specifically in any publications.