Monkey IL-12 p70 ELISA试剂盒(Interleukin-12 p70) (ab119533)

概述

  • 产品名称Monkey IL-12 p70 ELISA试剂盒(Interleukin-12 p70)
    参阅全部 IL12 p70 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 5%
    批次间
    样品 n Mean SD CV%
    Overall 4.8%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    2.6 U/ml
  • 范围
    3.9 U/ml - 250 U/ml
  • 复检

    105 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s IL-12 p70 (Interleukin-12 p70) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-12 p70 concentrations in Cell culture supernatant and serum (baboon).

    Monkey IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Monkey IL-12 p70 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Monkey IL-12 p70 captured on the plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 序列相似性Belongs to the IL-6 superfamily.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all

应用

Our Abpromise guarantee covers the use of ab119533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Monkey IL-12 p70 ELISA Kit (Interleukin-12 p70) 图像

  • Representative Standard Curve using ab119533.

     

实验方案

Monkey IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119533)参考文献

ab119533 has not yet been referenced specifically in any publications.

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Thank you very much for contacting us. Please find below the response from the lab:

We have not tested our ELISA in high salt Tris-based buffers and thus cannot tell you with a certainty how this buffer will affect the assay.

High sal...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"