Monkey IL-10 ELISA试剂盒(Interleukin-10) (ab119530)

概述

  • 产品名称Monkey IL-10 ELISA试剂盒(Interleukin-10)
    参阅全部 IL10 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 6 < 5%
    批次间
    样品 n Mean SD CV%
    Overall 6 < 10%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1.3 U/ml
  • 范围
    3.9 U/ml - 250 U/ml
  • 复检

    95 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s IL-10 (Interleukin-10) Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-10 in cell culture supernatant and serum (baboon, cynomolgus).

    This assay employs an antibody specific for Monkey IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Monkey IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 序列相似性Belongs to the IL-10 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • CSIF
    • Cytokine synthesis inhibitory factor
    • GVHDS
    • IL 10
    • IL-10
    • IL10
    • IL10_HUMAN
    • IL10A
    • Interleukin 10
    • Interleukin-10
    • Interleukin10
    • MGC126450
    • MGC126451
    • T cell growth inhibitory factor
    • TGIF
    see all

应用

Our Abpromise guarantee covers the use of ab119530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Monkey IL-10 ELISA Kit (Interleukin-10) 图像

  • Representative Standard Curve using ab119530.

实验方案

Monkey IL-10 ELISA Kit (Interleukin-10) (ab119530)参考文献

ab119530 has not yet been referenced specifically in any publications.

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