Monkey ICAM1 (CD54) ELISA试剂盒(ab119527)

概述

  • 产品名称Monkey ICAM1 (CD54) ELISA试剂盒
    参阅全部 ICAM1 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 5%
    批次间
    样品 n Mean SD CV%
    Overall < 10%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1.1 U/ml
  • 范围
    6.3 U/ml - 100 U/ml
  • 复检

    95 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s ICAM1 (CD54) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey ICAM1 (CD54) concentrations in cell culture supernatant and serum (baboon, rhesus macaque).

    ICAM1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated ICAM1 detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount ICAM1 captured on the plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
  • 细胞定位Membrane
  • 别名
    • BB2
    • CD 54
    • CD54
    • CD54 antigen
    • Cell surface glycoprotein P3.58
    • Human rhinovirus receptor
    • ICAM 1
    • Intercellular adhesion molecule 1
    • Major group rhinovirus receptor
    • MALA2
    • MGC6195
    • MyD10
    • P3.58
    • Surface antigen of activated B cells
    see all

应用

Our Abpromise guarantee covers the use of ab119527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Monkey ICAM1 (CD54) ELISA Kit 图像

  • Representative Standard Curve using ab119527.

实验方案

Monkey ICAM1 (CD54) ELISA Kit (ab119527)参考文献

ab119527 has not yet been referenced specifically in any publications.

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