Monkey E-selectin ELISA试剂盒(ab119523)

概述

  • 产品名称Monkey E-selectin ELISA试剂盒
    参阅全部 E-selectin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4 < 5%
    批次间
    样品 n Mean SD CV%
    Overall 4 < 10%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.4 U/ml
  • 范围
    1.9 U/ml - 60 U/ml
  • 复检

    85 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s E-selectin monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of monkey E-selectin concentrations in cell culture supernatant and serum.

    E-selectin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated E-selectin detection antibody. The microplate is then incubated at room temperature. After the removal of unbound proteins by washing TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of E-selectin captured on the plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 序列相似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD62 antigen-like family member E
    • CD62E
    • E-selectin
    • ELAM
    • ELAM-1
    • ELAM1
    • Endothelial adhesion molecule 1
    • Endothelial leukocyte adhesion molecule 1
    • ESEL
    • LECAM2
    • Leukocyte-endothelial cell adhesion molecule 2
    • LYAM2_HUMAN
    • Sele
    • Selectin E
    see all

应用

Our Abpromise guarantee covers the use of ab119523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Monkey E-selectin ELISA Kit 图像

  • Representative Standard Curve using ab119523.

实验方案

Monkey E-selectin ELISA Kit (ab119523)参考文献

ab119523 has not yet been referenced specifically in any publications.

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The product has 120 days guarantee period in Thailand. The 120 days starts form the date of purchase. Expiry date for biological compounds is difficult to guess so we provide guarantee rather than expiry date.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"