概述

  • 产品名称
    Monkey CD62P ELISA试剂盒
    参阅全部 CD62P 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4 < 10%
    批次间
    样品 n Mean SD CV%
    Overall 4 < 10%
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    1.3 U/ml
  • 范围
    0.8 U/ml - 50 U/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 76 % - %

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s CD62P Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey CD62P concentrations in cell culture supernatant, and serum (baboon).

    CD62P specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated CD62P detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the CD62P captured in the plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 2 units
    HRP-Conjugate anti-monkey CD62P monoclonal antibody 1 x 60µl
    Microplate coated with monoclonal antibody to monkey CD62P (12 x 8 wells) 1 unit
    Monkey CD62P Standard Lyophilized (100 U/mL upon reconstitution) 2 vials
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • 组织特异性
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • 疾病相关
    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • 序列相似性
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • Antigen CD62
    • CD62
    • CD62 antigen like family member P
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP 140
    • GMP-140
    • GMP140
    • Granule membrane protein 140
    • Granule membrane protein 140kDa
    • Granulocyte membrane protein
    • GRMP
    • LECAM3
    • Leukocyte endothelial cell adhesion molecule 3
    • Leukocyte-endothelial cell adhesion molecule 3
    • LYAM3_HUMAN
    • P selectin
    • P-selectin
    • PADGEM
    • Platelet activation dependent granule external membrane protein
    • Platelet activation dependent granule-external membrane protein
    • Platelet alpha granule membrane protein
    • PSEL
    • Selectin P
    • Selectin P (granule membrane protein 140kDa antigen CD62)
    • SELP
    see all

应用

Our Abpromise guarantee covers the use of ab119554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119554.

实验方案

文献

ab119554 has not yet been referenced specifically in any publications.

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