Monkey CD31 (PECAM-1) ELISA试剂盒(ab119552)

概述

  • 产品名称Monkey CD31 (PECAM-1) ELISA试剂盒
    参阅全部 CD31 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 2%
    批次间
    样品 n Mean SD CV%
    Overall < 10%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.06 U/ml
  • 范围
    0.48 U/ml - 30 U/ml
  • 复检

    110 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Monkey
  • 产品概述

    Abcam’s CD31 (PECAM-1) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey CD31 concentrations in cell culture supernatant and serum (baboon).

    CD31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an biotinylated-CD31 detection antibody. The microplate is then incubated at room temperature. Unbound conjugates are then washed away and TMB is added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD31 amount of sample captured in plate.

  • 说明

    Standard is recombinant protein produced in E.coli.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 别名
    • CD31
    • CD31 antigen
    • EndoCAM
    • GPIIA
    • GPIIA''
    • HEC7
    • Leukocyte surface protein
    • PECA1
    • PECA1_HUMAN
    • PECAM 1
    • PECAM-1
    • Pecam1
    • Platelet endothelial cell adhesion molecule
    • Platelet/endothelial cell adhesion molecule
    see all

应用

Our Abpromise guarantee covers the use of ab119552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Monkey CD31 (PECAM-1) ELISA Kit 图像

  • Representative Standard Curve using ab119552.

实验方案

Monkey CD31 (PECAM-1) ELISA Kit (ab119552)参考文献

ab119552 has not yet been referenced specifically in any publications.

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