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Abcam’s CD31 (PECAM-1) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey CD31 concentrations in cell culture supernatant and serum (baboon).
CD31 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with an biotinylated-CD31 detection antibody. The microplate is then incubated at room temperature. Unbound conjugates are then washed away and TMB is added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the CD31 amount of sample captured in plate.
Standard is recombinant protein produced in E.coli.
|组件||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate anti-Monkey CD31 monoclonal antibody||2 x 5µl|
|Microplate coated with monoclonal antibody to Monkey CD31 (12 x 8 wells)||1 unit|
|Monkey CD31 Standard (60 U/mL)||2 vials|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119552 has not yet been referenced specifically in any publications.