Anti-Mohawk homeobox抗体(ab66939)

概述

  • 产品名称Anti-Mohawk homeobox抗体
    参阅全部 Mohawk homeobox 一抗
  • 描述
    兔多克隆抗体to Mohawk homeobox
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: EGYSTPAHHT VIKGESSAIK AGGRPESRAA EDYVSPPKYK SSLLNRYLND, corresponding to internal sequence amino acids 181-230 of Mouse Mohawk homeobox

  • 阳性对照
    • NIH 3T3 lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab66939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May act as a morphogenetic regulator of cell adhesion.
  • 序列相似性Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9430023B20Rik antibody
    • C10orf48 antibody
    • Homeobox protein Mohawk antibody
    • IFRX antibody
    • Iroquois family related homeodomain protein antibody
    • iroquois homeobox protein like 1 antibody
    • IRXL1 antibody
    • MGC39616 antibody
    • MKX antibody
    • MKX_HUMAN antibody
    • mohawk homeobox antibody
    • OTTHUMP00000019374 antibody
    see all

Anti-Mohawk homeobox antibody 图像

  • Anti-Mohawk homeobox antibody (ab66939) at 2.5 µg/ml + NIH 3T3 lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : ~75 kDa. We are unsure as to the identity of these extra bands.

Anti-Mohawk homeobox antibody (ab66939)参考文献

ab66939 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory Stem Cells of Rabbit
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

提交于 Jul 22 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Olfactory Stem Cells (GFP))
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory Stem Cells (GFP)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

提交于 May 31 2016

Application Western blot
Sample Horse Cell lysate - whole cell (various cell type)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification various cell type
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization Yes - 0,1 % Triton X100
Specification Olfactory stem cells (tenocytes differentiation)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Antoine Veron

Verified customer

提交于 Aug 12 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Olfactory stem cells (differentiation tendon))
Permeabilization Yes - 0,1% Triton 100X
Specification Olfactory stem cells (differentiation tendon)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Antoine Veron

Verified customer

提交于 Jun 19 2015

Thank you for contacting us and your interest in our products.

As you have seen from our website, the anti-OAF antibody (ab116332) has not as yet been used in detecting themouse protein as you are hoping to do. This does not mean that it woul...

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