Anti-Mohawk homeobox抗体(ab66939)

概述

  • 产品名称
    Anti-Mohawk homeobox抗体
    参阅全部 Mohawk homeobox 一抗
  • 描述
    兔多克隆抗体to Mohawk homeobox
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: EGYSTPAHHT VIKGESSAIK AGGRPESRAA EDYVSPPKYK SSLLNRYLND, corresponding to internal sequence amino acids 181-230 of Mouse Mohawk homeobox

  • 阳性对照
    • NIH 3T3 lysate

性能

应用

Our Abpromise guarantee covers the use of ab66939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May act as a morphogenetic regulator of cell adhesion.
  • 序列相似性
    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 9430023B20Rik antibody
    • C10orf48 antibody
    • Homeobox protein Mohawk antibody
    • IFRX antibody
    • Iroquois family related homeodomain protein antibody
    • iroquois homeobox protein like 1 antibody
    • IRXL1 antibody
    • MGC39616 antibody
    • MKX antibody
    • MKX_HUMAN antibody
    • mohawk homeobox antibody
    • OTTHUMP00000019374 antibody
    see all

图片

  • Anti-Mohawk homeobox antibody (ab66939) at 2.5 µg/ml + NIH 3T3 lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : ~75 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab66939 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells of Rabbit
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

提交于 Jul 22 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Olfactory Stem Cells (GFP))
Permeabilization
Yes - 0,1% Triton X100
Specification
Olfactory Stem Cells (GFP)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Formaldehyde
Username

Antoine Veron

Verified customer

提交于 May 31 2016

Application
Western blot
Sample
Horse Cell lysate - whole cell (various cell type)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
various cell type
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization
Yes - 0,1 % Triton X100
Specification
Olfactory stem cells (tenocytes differentiation)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

提交于 Aug 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (Olfactory stem cells (differentiation tendon))
Permeabilization
Yes - 0,1% Triton 100X
Specification
Olfactory stem cells (differentiation tendon)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Antoine Veron

Verified customer

提交于 Jun 19 2015

Thank you for contacting us and your interest in our products.

As you have seen from our website, the anti-OAF antibody (ab116332) has not as yet been used in detecting themouse protein as you are hoping to do. This does not mean that it woul...

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