Anti-MOGT1抗体(ab81177)

概述

  • 产品名称
    Anti-MOGT1抗体
  • 描述
    兔多克隆抗体to MOGT1
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide designed within C terminal amino acids 288-337 (PIPVRQTLNP TQEQIEELHQ TYMEELRKLF EEHKGKYGIP EHETLVLK) of Human MOGT1 (NP_477513)

  • 阳性对照
    • Fetal liver lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

靶标

  • 功能
    Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine.
  • 通路
    Glycerolipid metabolism; triacylglycerol biosynthesis.
  • 序列相似性
    Belongs to the diacylglycerol acyltransferase family.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 0610030A14Rik antibody
    • 1110064N14Rik antibody
    • 2-acylglycerol O-acyltransferase 1 antibody
    • Acyl CoA:monoacylglycerol acyltransferase 1 antibody
    • Acyl-CoA:monoacylglycerol acyltransferase 1 antibody
    • DGAT2L antibody
    • DGAT2L1 antibody
    • Diacylglycerol acyltransferase 2 like antibody
    • Diacylglycerol acyltransferase 2-like protein 1 antibody
    • Diacylglycerol O acyltransferase 2 like 1 antibody
    • Diacylglycerol O-acyltransferase candidate 2 antibody
    • hDC2 antibody
    • mDC2 antibody
    • MGAT1 antibody
    • MGC118035 antibody
    • MOGAT1 antibody
    • MOGT1_HUMAN antibody
    • Monoacylglycerol O acyltransferase 1 antibody
    • Monoacylglycerol O-acyltransferase 1 antibody
    • OTTMUSP00000025181 antibody
    see all

图片

  • Anti-MOGT1 antibody (ab81177) at 1 µg/ml + fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab81177 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (stomach)
Gel Running Conditions
Reduced Denaturing
Loading amount
60 µg
Treatment
RIPA buffer is used for lysis buffer
Specification
stomach
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Jul 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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