纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
功能Probably involved in connections of major cytoskeletal structures to the plasma membrane.
组织特异性In all tissues and cultured cells studied.
序列相似性Contains 1 FERM domain.
翻译后修饰Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
细胞定位Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
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Anti-Moesin (phospho T558) antibody 图像
Western blot - Moesin (phospho T558) antibody (ab61109)
All lanes : Anti-Moesin (phospho T558) antibody (ab61109) at 1/500 dilution
Lane 1 : NIH/3T3 Jurkat Cells Lane 2 : NIH/3T3 Jurkat Cells with immunising Human Moesin Phosphopeptide
Predicted band size : 68 kDa Observed band size : 68 kDa
Anti-Moesin (phospho T558) antibody (ab61109)参考文献
This product has been referenced in:
Cooray SN et al. Ligand-specific conformational change of the G-protein-coupled receptor ALX/FPR2 determines proresolving functional responses. Proc Natl Acad Sci U S A110:18232-7 (2013).
Read more (PubMed: 24108355) »