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Purified bovine uterine moesin
The antibody has been used to inhibit both the binding of proteoheparan sulfate to smooth muscle cells, and the infectivity of measles virus.
Our Abpromise guarantee covers the use of ab50007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|Indirect ELISA||Use at an assay dependent concentration.|
|Dot blot||Use a concentration of 0.5 - 1 µg/ml.|
Immunohistochemistry (PFA-fixed) analysis of adult mouse brain tissue labelling Moesin with ab50007. Moesin is shown to be expressed in the adult RMS (rostral migratory stream). Top - low magnification image from DAB staining shows staining along the RMS. Bottom - confocal analysis of doublelabeling with PSA-NCAM shows expression pattern at the cellular level. Moesin was expressed in a number of PSA-NCAM positive cells of the RMS.
Brains were sagitally cut on a sliding microtome at 20μm. Immunostaining was preceded by antigen retrieval in sodium citrate, pH 6.0, for 20 min at 98°C followed by 30 minutes cooling at room temperature. For diaminobenzidine (DAB) staining, sections were washed in tris buffered saline (TBS), incubated for 30 minutes in 0.6% H2O2, blocked for 30 minutes with 3% normal donkey serum in 0.1% Triton X-100, and then incubated in primary antibodies for 48 hours at 4°C.
For fluorescent immunolabeling, free-floating sections were blocked for 30 minutes in 3% normal donkey serum in 0.1% Triton X-100, and then incubated for 48 hours at 4°C in primary antibody. After rinsing in TBS, sections were incubated for 1 hour with biotinylated donkey anti-mouse IgG. Sections were then washed and incubated with Alexa Fluor-conjugated secondary antibodies. ToPro3 was used as a nuclear counterstain.
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