概述

  • 产品名称
    Anti-MOBKL2C抗体
    参阅全部 MOBKL2C 一抗
  • 描述
    兔多克隆抗体to MOBKL2C
  • 特异性
    Detects endogenous levels of total MOBKL2C protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide from an internal region of human MOL2C.

  • 阳性对照
    • RAW264.7 cell extract K562 cell extract

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 26 kDa).
ELISA 1/40000.

靶标

  • 相关性
    MOBKL2C is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
  • 数据库链接
  • 别名
    • MOB kinase activator 3C antibody
    • Mob1 homolog 2C antibody
    • MOB1E antibody
    • MOB3C antibody
    • Mps One Binder kinase activator like 2C antibody
    see all

Anti-MOBKL2C antibody 图像

  • All lanes : Anti-MOBKL2C antibody (ab72756) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : K562 cell extract
    Lane 3 : RAW264.7 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 26 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 62 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-MOBKL2C antibody (ab72756)参考文献

ab72756 has not yet been referenced specifically in any publications.

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