Anti-MNK2抗体(ab84345)

概述

  • 产品名称Anti-MNK2抗体
    参阅全部 MNK2 一抗
  • 描述
    兔多克隆抗体to MNK2
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (SDFGLQCSAR PDMPASQPID IPDAKKRGKK KKRGRATDSF SGRFEDVYQL) of human MNK2 (NP_951009).

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab84345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • 组织特异性Ubiquitously expressed in all tissues examined. Isoform 2 is expressed at higher levels in the ovary than is isoform 1.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Dual phosphorylation of Thr-244 and Thr-249 activates the kinase. Phosphorylation of Thr-379 activates the kinase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G protein coupled receptor kinase 7 antibody
    • GPRK7 antibody
    • MAP kinase interacting serine threonine kinase 2 antibody
    • MAP kinase interacting serine threonine kinase antibody
    • MAP kinase interacting serine threonine protein kinase 2 antibody
    • MAP kinase signal integrating kinase 2 antibody
    • MAP kinase signal-integrating kinase 2 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 2 antibody
    • MAPK interacting kinase 2 antibody
    • MAPK signal integrating kinase 2 antibody
    • Mitogen activated protein kinase interacting serine threonine kinase 2 antibody
    • Mknk2 antibody
    • MKNK2_HUMAN antibody
    • Mnk2 antibody
    • Putative map kinase interacting kinase antibody
    see all

Anti-MNK2 antibody 图像

  • Anti-MNK2 antibody (ab84345) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ICC/IF image of ab84345 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84345, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab84345 at 5 µg/ml staining MNK2 in Human Placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84345 at 5 µg/ml staining MNK2 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • IHC image of ab84345 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84345, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-MNK2 antibody (ab84345) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 10 µg
    Lane 4 : Zebrafish liver homogenate at 10 µg
    Lane 5 : Zebrafish skeletal muscle homogenate at 10 µg
    Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa


    Exposure time : 5 minutes

Anti-MNK2 antibody (ab84345)参考文献

ab84345 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.The immunogen sequence for ab84345 is available on the public website and so I blasted this sequence against the human genome and it showed that the immunogen had 100% homology to both isoforms 2a and 2b of MNK2.As to the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"