RabMAb

Anti-MNK1 (phospho T385)抗体[EPR2370] (ab109102)

概述

  • 产品名称Anti-MNK1 (phospho T385)抗体[EPR2370]
    参阅全部 MNK1 一抗
  • 描述
    兔单克隆抗体[EPR2370] to MNK1 (phospho T385)
  • 特异性ab109102 recognises MNK1 only when phosphorilated on Threonine 385.
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho synthetic peptide corresponding to residues in the region of T385 of Human MNK1.

  • 阳性对照
    • HeLa cell lysates, treated with Calyculin A; Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • 翻译后修饰Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
    • 细胞定位Cytoplasm and Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MAP kinase interacting kinase 1 antibody
      • MAP kinase interacting serine/threonine kinase 1 antibody
      • MAP kinase signal integrating kinase 1 antibody
      • MAP kinase signal-integrating kinase 1 antibody
      • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
      • MAPK signal integrating kinase 1 antibody
      • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
      • mknk1 antibody
      • MKNK1_HUMAN antibody
      • MNK 1 antibody
      • Mnk1 antibody
      see all

    Anti-MNK1 (phospho T385) antibody [EPR2370] 图像

    • All lanes : Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 51 kDa
    • Immunohistochemical analysis of MNK1 (phospho T385) expression in paraffin-embedded Human colon tissue using ab109102 at 1/250 dilution.

    Anti-MNK1 (phospho T385) antibody [EPR2370] (ab109102)参考文献

    This product has been referenced in:
    • Attar-Schneider O  et al. Migration and epithelial-to-mesenchymal transition of lung cancer can be targeted via translation initiation factors eIF4E and eIF4GI. Lab Invest 96:1004-15 (2016). Read more (PubMed: 27501049) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"