Anti-MNK1 (phospho T250)抗体(ab111434)

概述

  • 产品名称Anti-MNK1 (phospho T250)抗体
    参阅全部 MNK1 一抗
  • 描述
    兔多克隆抗体to MNK1 (phospho T250)
  • 特异性ab111434 detects endogenous levels of MNK1 only when phosphorylated at threonine 250 (Human: Thr250; Mouse: Thr209; Rat: Thr197).
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human MNK1 around the phosphorylation site of threonine 250 (I-T-TP-P-E).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab111434 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种���IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab111434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
  • 细胞定位Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MAP kinase interacting kinase 1 antibody
    • MAP kinase interacting serine/threonine kinase 1 antibody
    • MAP kinase signal integrating kinase 1 antibody
    • MAP kinase signal-integrating kinase 1 antibody
    • MAP kinase-interacting serine/threonine-protein kinase 1 antibody
    • MAPK signal integrating kinase 1 antibody
    • MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1 antibody
    • mknk1 antibody
    • MKNK1_HUMAN antibody
    • MNK 1 antibody
    • Mnk1 antibody
    see all

Anti-MNK1 (phospho T250) antibody 图像

  • ab111434, at 1/50 dilution, staining MNK1 in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-MNK1 (phospho T250) antibody (ab111434)参考文献

ab111434 has not yet been referenced specifically in any publications.

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