概述

  • 产品名称Anti-MMP9抗体[SB15c]
    参阅全部 MMP9 一抗
  • 描述
    小鼠单克隆抗体[SB15c] to MMP9
  • 特异性This antibody does not cross react with human MMP1, MMP2 or MMP3.
  • 经测试应用适用于: ELISA, IHC-P, Sandwich ELISA, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein;Human MMP9

  • 阳性对照
    • Human colon tissue.

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应用

Our Abpromise guarantee covers the use of ab51203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/3000 - 1/8000.
IHC-P Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MMP9 (ab38902). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab38902 as Detection.
ICC Use at an assay dependent concentration.

靶标

  • 功能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 组织特异性Produced by normal alveolar macrophages and granulocytes.
  • 疾病相关Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • 细胞定位Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody [SB15c] 图像

  • This image shows human colon tissue stained with ab51203 at 5µg/ml.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-small-cell lung cancer tissue staining MMP9 with ab51203.

    Antigen retrieval was performed by placing the sections in 0.01 M citrate buffer (pH 6) and heating in a microwave oven. Samples were incubated with primary antibody (1/100) overnight at 4°C. Staining was detected using an IHC substrate kit.
  • Standard curve for MMP9 (Analyte: ab82955); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [SB15c] to MMP9 (ab51203) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP9 - Hinge region (ab38902) at 0.5µg/ml.

实验方案

Anti-MMP9 antibody [SB15c] (ab51203)参考文献

This product has been referenced in:
  • Liu GH  et al. MicroRNA-524-5p suppresses the growth and invasive abilities of gastric cancer cells. Oncol Lett 11:1926-1932 (2016). WB ; Human . Read more (PubMed: 26998102) »
  • Stenvold H  et al. Overexpression of matrix metalloproteinase-7 and -9 in NSCLC tumor and stromal cells: correlation with a favorable clinical outcome. Lung Cancer 75:235-41 (2012). IHC-P ; Human . Read more (PubMed: 21764478) »

See all 3 Publications for this product

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