Anti-MMP9抗体- Carboxyterminal end (ab38906)

概述

  • 产品名称Anti-MMP9抗体- Carboxyterminal end
    参阅全部 MMP9 一抗
  • 描述
    兔多克隆抗体to MMP9 - Carboxyterminal end
  • 特异性This antibody binds to both reduced and non reduced MMP9 proteins. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3).
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.
    (Peptide available as ab41067)

  • 阳性对照
    • Cell media from human chondrosarcoma.

性能

应用

Our Abpromise guarantee covers the use of ab38906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 95 kDa.

WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Detects a band of approximately 95 kDa. Dilution optimised using Chromogenic detection.

Flow Cyt 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 组织特异性Produced by normal alveolar macrophages and granulocytes.
  • 疾病相关Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • 细胞定位Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody - Carboxyterminal end 图像

  • All lanes : Anti-MMP9 antibody - Carboxyterminal end (ab38906) at 1/1000 dilution

    Lane 1 : Human MMP9
    Lane 2 : Cell media from human chondrosarcoma (untreated)


    Observed band size : 97 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab38906 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38906, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MMP9 antibody - Carboxyterminal end (ab38906)参考文献

This product has been referenced in:
  • Lluri G  et al. Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro. Exp Cell Res 314:11-24 (2008). WB, ICC/IF ; Mouse . Read more (PubMed: 17678891) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (U87MG human Astrocytoma cells)
Specification U87MG human Astrocytoma cells
Fixation Paraformaldehyde
Permeabilization No
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提交于 Apr 10 2013

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