概述

  • 产品名称Anti-MMP9抗体[2C3]
    参阅全部 MMP9 一抗
  • 描述
    小鼠单克隆抗体[2C3] to MMP9
  • 特异性ab53296 targets MMP9.
  • 经测试应用适用于: WB, Inhibition Assay, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    KLGLGADVAQVT

    conjugated to Ovalbumin, corresponding to C terminal amino acids 603-614 of Human MMP9

应用

Our Abpromise guarantee covers the use of ab53296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 78 kDa.
Inhibition Assay Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 组织特异性Produced by normal alveolar macrophages and granulocytes.
  • 疾病相关Intervertebral disc disease
    Metaphyseal anadysplasia 2
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • 细胞定位Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 82 kDa matrix metalloproteinase-9 antibody
    • 92 kDa gelatinase antibody
    • 92 kDa type IV collagenase antibody
    • CLG 4B antibody
    • CLG4B antibody
    • Collagenase Type 4 beta antibody
    • Collagenase type IV 92 KD antibody
    • EC 3.4.24.35 antibody
    • Gelatinase 92 KD antibody
    • Gelatinase B antibody
    • Gelatinase beta antibody
    • GelatinaseB antibody
    • GELB antibody
    • Macrophage gelatinase antibody
    • MANDP2 antibody
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 9 antibody
    • MMP 9 antibody
    • MMP-9 antibody
    • MMP9 antibody
    • MMP9_HUMAN antibody
    • Type V collagenase antibody
    see all

Anti-MMP9 antibody [2C3] (ab53296)参考文献

This product has been referenced in:
  • Hackel D  et al. Transient opening of the perineurial barrier for analgesic drug delivery. Proc Natl Acad Sci U S A 109:E2018-27 (2012). Rat . Read more (PubMed: 22733753) »
  • Radjabi AR  et al. Thrombin Induces Tumor Invasion through the Induction and Association of Matrix Metalloproteinase-9 and {beta}1-Integrin on the Cell Surface. J Biol Chem 283:2822-34 (2008). Inhibition Assay ; Human . Read more (PubMed: 18048360) »

See all 3 Publications for this product

Product Wall



I can confirm that ab53296 Anti-MMP9 antibody [2C3] antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concen...

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I confirmed with the lab and am happy to let you know that the datasheet is correct and this antibody does not contain any sodium azide nor does it contain any other preservative.

Thank you for your message. I notice from our records that you have been waiting a long time for a resonse and Isincerely apologise for the long delay. Iappreciate your patience.

I contacted the originator and regrettably they have taken a l...

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Thank you for your enquiry.

We do have a large range of isotype control antibodies which can be found on the following page from our website:

http://www.abcam.com/index.html?c=4161

This includes several Mouse monoclonal...

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Thank you for your enquiry.

I can confirm that ab53296MMP9 antibody [2C3] is sold astissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"