概述

  • 产品名称Anti-MMP9抗体[15W2]
    参阅全部 MMP9 一抗
  • 描述
    小鼠单克隆抗体[15W2] to MMP9
  • 特异性This antibody is specific to MMP9. It does not crossreact with MMPs 1, 2, 3 and 10 as determined by ELISA.
  • 经测试应用适用于: IHC-Fr, IHC-Pmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Prokaryotic recombinant protein corresponding to a 134 amino acid portion of the C-terminal region of the mature human MMP9 molecule.

  • 阳性对照
    • Liver tissue.

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应用

Our Abpromise guarantee covers the use of ab49478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/20 - 1/40. Acetone fixation is recommended.
IHC-P 1/40 - 1/80. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
      -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
    • 组织特异性Produced by normal alveolar macrophages and granulocytes.
    • 疾病相关Intervertebral disc disease
      Metaphyseal anadysplasia 2
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin repeats.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
      N- and O-glycosylated.
    • 细胞定位Secreted, extracellular space, extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 82 kDa matrix metalloproteinase-9 antibody
      • 92 kDa gelatinase antibody
      • 92 kDa type IV collagenase antibody
      • CLG 4B antibody
      • CLG4B antibody
      • Collagenase Type 4 beta antibody
      • Collagenase type IV 92 KD antibody
      • EC 3.4.24.35 antibody
      • Gelatinase 92 KD antibody
      • Gelatinase B antibody
      • Gelatinase beta antibody
      • GelatinaseB antibody
      • GELB antibody
      • Macrophage gelatinase antibody
      • MANDP2 antibody
      • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 9 antibody
      • MMP 9 antibody
      • MMP-9 antibody
      • MMP9 antibody
      • MMP9_HUMAN antibody
      • Type V collagenase antibody
      see all

    Anti-MMP9 antibody [15W2] 图像

    • ab49478 staining MMP9 in human oesophagus.
      Left panel: with primary antibody diluted at 1:1000. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    Anti-MMP9 antibody [15W2] (ab49478)参考文献

    This product has been referenced in:
    • Monaghan H  et al. The role of matrix metalloproteinases-2, -7 and -9 and beta-catenin in high grade endometrial carcinoma. Histopathology 50:348-57 (2007). Read more (PubMed: 17257130) »

    See 1 Publication for this product

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