概述

  • 产品名称Anti-MMP8抗体
    参阅全部 MMP8 一抗
  • 描述
    兔多克隆抗体to MMP8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human MMP8.

    (Peptide available as ab94385.)

  • 阳性对照
    • This antibody gave a positive signal in the following human lysates: HeLa whole cell; bone marrow tissue; skin tissue.

性能

应用

Our Abpromise guarantee covers the use of ab72897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Can degrade fibrillar type I, II, and III collagens.
    • 组织特异性Neutrophils.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CLG 1 antibody
      • CLG1 antibody
      • Collagenase 1 antibody
      • Collagenase 1 neutrophil antibody
      • HNC antibody
      • Matrix metallopeptidase 8 (neutrophil collagenase) antibody
      • Matrix metalloprotease 8 antibody
      • Matrix metalloproteinase-8 antibody
      • MMP 8 antibody
      • MMP-8 antibody
      • Mmp8 antibody
      • MMP8_HUMAN antibody
      • Neutrophil collagenase antibody
      • PMNL CL antibody
      • PMNL collagenase antibody
      • PMNL-CL antibody
      • PMNLCL antibody
      see all

    Anti-MMP8 antibody 图像

    • All lanes : Anti-MMP8 antibody (ab72897) at 1 µg/ml

      Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : Human skin tissue lysate - total protein (ab30166)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes

    Anti-MMP8 antibody (ab72897)参考文献

    ab72897 has not yet been referenced specifically in any publications.

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