The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Also detects a band at approximately 22 kDa.
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Belongs to the peptidase M10A family.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Secreted > extracellular space > extracellular matrix.
All lanes : Anti-MMP7 antibody (ab85144) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Human placenta tissue lysate - total protein (ab29745) Lane 3 : Human liver tissue lysate - total protein (ab29889) Lane 4 : Human kidney tissue lysate - total protein (ab30203) Lane 5 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
MMP7 protein contains both a 17-amino acid signal sequence and an activation peptide (77 amino-acids) (Swissprot). If both were removed, the expected MW would be around 20-kDa. We hypothesise that the band at approximately 20-kDa, detected in A431 whole cell lysate, and in human liver, kidney and small intestine tissue lysates, is the cleaved form of MMP7. The band at approximately 29-kDa in human placenta tissue lysate is probably the full length protein.
IHC image of MMP7 staining in human pancreatic cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85144, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX