概述

  • 产品名称
  • 描述
    兔多克隆抗体to MMP7
  • 特异性
    This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.
  • 经测试应用
    适用于: WB, ELISA, IP, IHC-Fr, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to C-Terminus of MMP7 (Human).

  • 阳性对照
    • PMA stimulated A431 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab5706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IP
IHC-Fr
ICC/IF
IHC-P
  • 应用说明
    ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use 3-5µg for 106 cells.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
    • 序列相似性
      Belongs to the peptidase M10A family.
    • 结构域
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    图片

    • ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    文献

    This product has been referenced in:
    • Mitsui Y  et al. Versican Promotes Tumor Progression, Metastasis and Predicts Poor Prognosis in Renal Carcinoma. Mol Cancer Res 15:884-895 (2017). Read more (PubMed: 28242813) »
    • Sonobe T  et al. Immediate and Long-Term Outcome of Acute H2S Intoxication Induced Coma in Unanesthetized Rats: Effects of Methylene Blue. PLoS One 10:e0131340 (2015). Read more (PubMed: 26115032) »

    See all 11 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (stomach cancer cell)
    Loading amount
    25 µg
    Specification
    stomach cancer cell
    Treatment
    10uM cannabinoid antagonist
    Gel Running Conditions
    Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 08 2013

    Thanks for your reply.

    If you can provide the PO number or Abcam order reference number associated with the purchase of ab5706 I will be happy to arrange shipment of ab4044 as a replacement.

    Thanks for the additional information!

    I look forward to seeing your next western blotwhere:

    1. Lesspure MMP7 is loaded as control

    2.Molecular weight markers are noted

    Thanks for sending along the blot image. Here are my thoughts:

    1) Where are the markers and marker labels?;

    2) Has any other antibody been used successfully with these samples?;

    3) It is mentioned 0.5mg protein loaded, Is ...

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    Thank you for submitting your completed questionnaire. I am sorry to hear this vial of ab5706 is giving you trouble.

    You mentioned including a western blot in your enquiry but unfortunately it did not make it through. If you could, please re ...

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    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

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    Thank you for your message and for kindly providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be plea...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this...

    Read More
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Pancreas)
    Specification
    Pancreas
    Blocking step
    Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% serum +
    Username

    Dr. Howard Crawford

    Verified customer

    提交于 Feb 21 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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