概述

  • 产品名称Anti-MMP7抗体
    参阅全部 MMP7 一抗
  • 描述
    兔多克隆抗体to MMP7
  • 特异性This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.
  • 经测试应用适用于: WB, ELISA, IP, IHC-Fr, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to C-Terminus of MMP7 (Human).

  • 阳性对照
    • PMA stimulated A431 whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab5706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IP
IHC-Fr
ICC/IF
IHC-P
  • 应用说明ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use 3-5µg for 106 cells.
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
    • 序列相似性Belongs to the peptidase M10A family.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Matrilysin antibody
      • Matrin antibody
      • Matrix Metalloproteinase 7 antibody
      • Matrix metalloproteinase-7 antibody
      • MMP 7 antibody
      • MMP-7 antibody
      • MMP7 antibody
      • MMP7_HUMAN antibody
      • MPSL1 antibody
      • PUMP 1 antibody
      • Pump 1 protease antibody
      • Pump-1 protease antibody
      • PUMP1 antibody
      • Uterine matrilysin antibody
      • Uterine metalloproteinase antibody
      see all

    Anti-MMP7 antibody 图像

    • The whole cell lysate derived from PMA-stimulated A431 was probed with rabbit anti MMP7 at 1/500 (Lane A) or by pre-absorption antibody with immunizing peptides (Lane B).
    • ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Anti-MMP7 antibody (ab5706)参考文献

    This product has been referenced in:
    • Sonobe T  et al. Immediate and Long-Term Outcome of Acute H2S Intoxication Induced Coma in Unanesthetized Rats: Effects of Methylene Blue. PLoS One 10:e0131340 (2015). Read more (PubMed: 26115032) »
    • Abbas A  et al. Matrix metalloproteinase 7 is associated with symptomatic lesions and adverse events in patients with carotid atherosclerosis. PLoS One 9:e84935 (2014). IHC-P, ICC/IF ; Human . Read more (PubMed: 24400123) »

    See all 9 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (stomach cancer cell)
    Loading amount 25 µg
    Specification stomach cancer cell
    Treatment 10uM cannabinoid antagonist
    Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 08 2013

    Thanks for your reply.

    If you can provide the PO number or Abcam order reference number associated with the purchase of ab5706 I will be happy to arrange shipment of ab4044 as a replacement.

    Thanks for the additional information!

    I look forward to seeing your next western blotwhere:

    1. Lesspure MMP7 is loaded as control

    2.Molecular weight markers are noted

    Thanks for sending along the blot image. Here are my thoughts:

    1) Where are the markers and marker labels?;

    2) Has any other antibody been used successfully with these samples?;

    3) It is mentioned 0.5mg protein loaded, Is ...

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    Thank you for submitting your completed questionnaire. I am sorry to hear this vial of ab5706 is giving you trouble.

    You mentioned including a western blot in your enquiry but unfortunately it did not make it through. If you could, please re ...

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    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

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    Thank you for your message and for kindly providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be plea...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investigate this...

    Read More
    Application Immunocytochemistry
    Sample Mouse Cell (Pancreas)
    Specification Pancreas
    Blocking step Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% serum +
    Username

    Dr. Howard Crawford

    Verified customer

    提交于 Feb 21 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"