概述

  • 产品名称
  • 描述
    兔多克隆抗体to MMP3
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MMP3.

    (Peptide available as ab73953.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human Tissue lysates: Heart, Liver, Lung

应用

Our Abpromise guarantee covers the use of ab63853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    IHC-P: 1/50 (See abreview).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • 序列相似性
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Proteoglycanase antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelysin 1 antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    图片

    • All lanes : Anti-MMP3 antibody (ab63853) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)
      Lane 3 : Lung (Human) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 54 kDa


      Exposure time : 10 minutes
    • ab63853 staining MMP3 in Human breast carcinoma tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 5 minutes of peroxidase block then 10 minutes of protein block. Antigen retrieval was by heat mediation in Retrieval solution. Samples were incubated with primary antibody (1/50) for 45 minutes at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

      See Abreview

    文献

    ab63853 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (U251, NIH-3T3, Ms Ovary, PC12)
    Loading amount
    20 µg
    Specification
    U251, NIH-3T3, Ms Ovary, PC12
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Feb 08 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast carcinoma)
    Specification
    Breast carcinoma
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Jan 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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