概述

  • 产品名称Anti-MMP3抗体
    参阅全部 MMP3 一抗
  • 描述
    兔多克隆抗体to MMP3
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 251 - 478 of Human MMP3.

  • 阳性对照
    • Conditioned, serum free medium from TPA treated human Endometrial cells. Human placenta tissue.

应用

Our Abpromise guarantee covers the use of ab32607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/50 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Proteoglycanase antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelysin 1 antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody 图像

    • Ab32607, at a 1/50 dilution, staining MMP3 in formalin fixed paraffin embedded human placenta tissue by Immunohistochemistry.

    Anti-MMP3 antibody (ab32607)参考文献

    This product has been referenced in:
    • Lee CW  et al. Nestin depletion induces melanoma matrix metalloproteinases and invasion. Lab Invest 94:1382-95 (2014). IF, IHC ; Mouse, Human . Read more (PubMed: 25365206) »
    • Järvinen TM  et al. Matrix metalloproteinases as mediators of tissue injury in different forms of cutaneous lupus erythematosus. Br J Dermatol 157:970-80 (2007). Read more (PubMed: 17854363) »

    See all 2 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (human fetal heart)
    Specification human fetal heart
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% saponin in PBS
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Dr. Silvia Martin-Puig

    Verified customer

    提交于 Apr 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"