概述

  • 产品名称
  • 描述
    兔多克隆抗体to MMP3
  • 经测试应用
    适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 251 - 478 of Human MMP3.

  • 阳性对照
    • Conditioned, serum free medium from TPA treated human Endometrial cells. Human placenta tissue.

应用

Our Abpromise guarantee covers the use of ab32607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明
    IHC-P: 1/50 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • 序列相似性
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Proteoglycanase antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelysin 1 antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody 图像

    • Ab32607, at a 1/50 dilution, staining MMP3 in formalin fixed paraffin embedded human placenta tissue by Immunohistochemistry.

    Anti-MMP3 antibody (ab32607)参考文献

    This product has been referenced in:
    • Lee CW  et al. Nestin depletion induces melanoma matrix metalloproteinases and invasion. Lab Invest 94:1382-95 (2014). IF, IHC ; Mouse, Human . Read more (PubMed: 25365206) »
    • Mäkitalo L  et al. Matrix metalloproteinases in the restorative proctocolectomy pouch of pediatric ulcerative colitis. World J Gastroenterol 18:4028-36 (2012). Read more (PubMed: 22912554) »

    See all 3 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (human fetal heart)
    Specification
    human fetal heart
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% saponin in PBS
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Dr. Silvia Martin-Puig

    Verified customer

    提交于 Apr 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册