概述

  • 产品名称
    Anti-MMP3抗体[4B7.3]
    参阅全部 MMP3 一抗
  • 描述
    小鼠单克隆抗体[4B7.3] to MMP3
  • 宿主
    Mouse
  • 特异性
    The antibody reacts with MMP3 (Stromelysin 1). It binds to both the reduced (the pro form as well as active forms) and the non reduced proteins. The antibody has been observerd to cross react with MMP10 (Stromelysin 2) but does not cross react with the other Stromelysin form (Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc.).
  • 经测试应用
    适用于: WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein of Human MMP3.

  • 阳性对照
    • Cell media from human chondrosarcoma (treated with TPA).
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

应用

Our Abpromise guarantee covers the use of ab38907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 59, 57 kDa (predicted molecular weight: 54 kDa). Detects bands of approximately 59 and 57 kDa (the pro form) when used against the reduced protein. Dilution optimised using Chromogenic detection.
Sandwich ELISA Use a concentration of 1 µg/ml.

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab38912 as Detection.

靶标

  • 功能
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 序列相似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
    • 别名
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Proteoglycanase antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelysin 1 antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    图片

    • Sandwich ELISA - Anti-MMP3 antibody [4B7.3] (ab38907)
      Sandwich ELISA - Anti-MMP3 antibody [4B7.3] (ab38907)
      Standard curve for MMP3 (Analyte: ab39306) ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [4B7.3] to MMP3 (ab38907) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP3 - Whole molecule (ab38912) at 0.5µg/ml.
    • Western blot - Anti-MMP3 antibody [4B7.3] (ab38907)
      Western blot - Anti-MMP3 antibody [4B7.3] (ab38907)
      All lanes : Anti-MMP3 antibody [4B7.3] (ab38907) at 1/1000 dilution

      Lane 1 : Human MMP3
      Lane 2 : Cell media from human chondrosarcoma (treated with TPA)

      Predicted band size: 54 kDa



      When used against the reduced protein, the antibody identifies bands at 59 kDa and 57 kDa (the pro form).

    文献

    This product has been referenced in:
    • Beltrami-Moreira M  et al. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling. PLoS One 11:e0152474 (2016). Read more (PubMed: 27032103) »
    • Sauls K  et al. Increased Infiltration of Extra-Cardiac Cells in Myxomatous Valve Disease. J Cardiovasc Dev Dis 2:200-213 (2015). IHC, ICC/IF ; Mouse . Read more (PubMed: 26473162) »

    See all 6 Publications for this product

    发表研究结果有使用 ab38907？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Western blot abreview for Anti-MMP3 antibody [4B7.3]

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    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Purified protein (Purified from cultured cells)
    Loading amount
    0.5 µg
    Specification
    Purified from cultured cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
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    提交于 Jul 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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