概述

  • 产品名称Anti-MMP3抗体[4B7.3]
    参阅全部 MMP3 一抗
  • 描述
    小鼠单克隆抗体[4B7.3] to MMP3
  • 特异性The antibody reacts with MMP3 (Stromelysin 1). It binds to both the reduced (the pro form as well as active forms) and the non reduced proteins. The antibody has been observerd to cross react with MMP10 (Stromelysin 2) but does not cross react with the other Stromelysin form (Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc.).
  • 经测试应用适用于: WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein of Human MMP3.

  • 阳性对照
    • Cell media from human chondrosarcoma (treated with TPA).

应用

Our Abpromise guarantee covers the use of ab38907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 59, 57 kDa (predicted molecular weight: 54 kDa). Detects bands of approximately 59 and 57 kDa (the pro form) when used against the reduced protein. Dilution optimised using Chromogenic detection.
Sandwich ELISA Use a concentration of 1 µg/ml. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab38912 as Detection.

靶标

  • 功能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

Anti-MMP3 antibody [4B7.3] 图像

  • Standard curve for MMP3 (Analyte: ab39306) ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [4B7.3] to MMP3 (ab38907) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP3 - Whole molecule (ab38912) at 0.5µg/ml.
  • All lanes : Anti-MMP3 antibody [4B7.3] (ab38907) at 1/1000 dilution

    Lane 1 : Human MMP3
    Lane 2 : Cell media from human chondrosarcoma (treated with TPA)


    Predicted band size : 54 kDa

Anti-MMP3 antibody [4B7.3] (ab38907)参考文献

This product has been referenced in:
  • Hirata H  et al. Role of secreted frizzled-related protein 3 in human renal cell carcinoma. Cancer Res 70:1896-905 (2010). WB ; Human . Read more (PubMed: 20160027) »
  • Pozgan U  et al. Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis. Biol Chem 391:571-9 (2010). Read more (PubMed: 20180636) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (Purified from cultured cells)
Loading amount 0.5 µg
Specification Purified from cultured cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 05 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"