重组
RabMAb

Anti-MMP20抗体[EP1275Y] (ab76109)

概述

  • 产品名称
    Anti-MMP20抗体[EP1275Y]
    参阅全部 MMP20 一抗
  • 描述
    兔单克隆抗体[EP1275Y] to MMP20
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human MMP20 aa 450-550 (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • HeLa cell lysate; HeLa cells.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
    • 组织特异性
      Expressed specifically in the enamel organ.
    • 疾病相关
      Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
    • 序列相似性
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰
      Autoactivates at least at the 107-Asn-
      -Tyr-108 site.
    • 细胞定位
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI2A2 antibody
      • Enamel metalloproteinase antibody
      • Enamelysin antibody
      • Matrix metalloproteinase 20 antibody
      • Matrix metalloproteinase-20 antibody
      • MMP 20 antibody
      • MMP-20 antibody
      • MMP20 antibody
      • MMP20_HUMAN antibody
      see all

    图片

    • ab76109 at 1/100-1/250 dilution staining MMP20 in HeLa cells by Immunofluorescence.
    • Anti-MMP20 antibody [EP1275Y] (ab76109) at 1/500 dilution + HeLa cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa

    文献

    This product has been referenced in:
    • Yang T  et al. High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2. Arch Oral Biol 58:1165-73 (2013). WB ; Mouse . Read more (PubMed: 23598055) »
    • Mohazab L  et al. Critical role for avß6 integrin in enamel biomineralization. J Cell Sci 126:732-44 (2013). Read more (PubMed: 23264742) »

    See all 2 Publications for this product

    客户评价及客户问答

    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit ab76109que vous avez reçu ne fonctionne pas comme attendu.
    Comme convenu ce matin par téléphone, j'ai mis en place l...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册