重组Anti-MMP20抗体[EP1275Y] (ab76109)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1275Y] to MMP20
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MMP20抗体[EP1275Y]
参阅全部 MMP20 一抗 -
描述
兔单克隆抗体[EP1275Y] to MMP20 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human MMP20 aa 450-550 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa whole cell lysate (ab150035); HeLa cells.
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常规说明
The production method for this product has been changed from hybridoma to recombinant on 15th April 2024
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1275Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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说明 |
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WB
1/500. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
靶标
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功能
Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation. -
组织特异性
Expressed specifically in the enamel organ. -
疾病相关
Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Autoactivates at least at the 107-Asn-
-Tyr-108 site. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 9313 Human
- Entrez Gene: 30800 Mouse
- Entrez Gene: 300341 Rat
- Omim: 604629 Human
- SwissProt: O60882 Human
- SwissProt: P57748 Mouse
- Unigene: 591946 Human
- Unigene: 390121 Mouse
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别名
- AI2A2 antibody
- Enamel metalloproteinase antibody
- Enamelysin antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab76109 被引用在 4 文献中.
- Liang Y et al. Effect of canonical NF-?B signaling pathway on the differentiation of rat dental epithelial stem cells. Stem Cell Res Ther 10:139 (2019). PubMed: 31109359
- Li Z et al. Bcl11b regulates enamel matrix protein expression and dental epithelial cell differentiation during rat tooth development. Mol Med Rep 15:297-304 (2017). PubMed: 27959403
- Yang T et al. High amounts of fluoride induce apoptosis/cell death in matured ameloblast-like LS8 cells by downregulating Bcl-2. Arch Oral Biol 58:1165-73 (2013). WB ; Mouse . PubMed: 23598055
- Mohazab L et al. Critical role for avß6 integrin in enamel biomineralization. J Cell Sci 126:732-44 (2013). PubMed: 23264742