Anti-MMP2抗体(ab97779)
Key features and details
- Rabbit polyclonal to MMP2
- Suitable for: IHC-P, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MMP2抗体
参阅全部 MMP2 一抗 -
描述
兔多克隆抗体to MMP2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IP, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Sheep, Rabbit, Cow, Pig, Xenopus laevis -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 193 and 545 of Human MMP2 (NP_004521).
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阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate; U-87 MG whole cell extracts. PC-3, mouse brain.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97779于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (2) |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/1000.
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. |
IP
1/100 - 1/500. |
ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
靶标
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功能
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. -
组织特异性
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. -
疾病相关
Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. -
序列相似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. -
细胞定位
Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 282872 Cow
- Entrez Gene: 4313 Human
- Entrez Gene: 397391 Pig
- Entrez Gene: 100009000 Rabbit
- Entrez Gene: 81686 Rat
- Entrez Gene: 380389 Xenopus laevis
- Omim: 120360 Human
- SwissProt: Q9GLE5 Cow
see all -
别名
- 72 kDa gelatinase antibody
- 72kD type IV collagenase antibody
- CLG 4 antibody
see all
图片
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Anti-MMP2 antibody (ab97779) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS Page -
All lanes : Anti-MMP2 antibody (ab97779) at 1/3000 dilution
Lane 1 : PC-3
Lane 2 : U-87 MG
Lane 3 : SK-N-SH
Lysates/proteins at 30 µg per lane.
Predicted band size: 74 kDa -
Immunohistochemical analysis of Human endometrical carcinoma labeling MMP2 with ab97779 at 1/500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MMP2 with ab97779 at 1/500 dilution.
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MMP2 was immunoprecipitated from U-87 MG (glioblastoma-astrocytoma epithelial cell line) whole cell extracts with 5 µg ab97779. Western blot was performed from the immunoprecipitate using ab97779.
Lane 1: Control IgG IP in U-87 MG whole cell extracts.
Lane 2: ab97779 IP in U-87 MG whole cell extracts.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (166)
ab97779 被引用在 166 文献中.
- Buchler A et al. Selective Imaging of Matrix Metalloproteinase-13 to Detect Extracellular Matrix Remodeling in Atherosclerotic Lesions. Mol Imaging Biol 24:93-103 (2022). PubMed: 34231104
- Chen H et al. Alterations of mRNA and lncRNA profiles associated with the extracellular matrix and spermatogenesis in goats. Anim Biosci 35:544-555 (2022). PubMed: 34530511
- Misilimu D et al. Intranasal Salvinorin A Improves Long-term Neurological Function via Immunomodulation in a Mouse Ischemic Stroke Model. J Neuroimmune Pharmacol 17:350-366 (2022). PubMed: 34596819
- Pan S et al. SOX4 induces drug resistance of colorectal cancer cells by downregulating CYLD through transcriptional activation of microRNA-17. J Biochem Mol Toxicol 36:e22910 (2022). PubMed: 34927777
- Hu J et al. Trimethylamine N-Oxide Promotes Abdominal Aortic Aneurysm Formation by Aggravating Aortic Smooth Muscle Cell Senescence in Mice. J Cardiovasc Transl Res 15:1064-1074 (2022). PubMed: 35143032