Anti-MMP2抗体(ab83173)

概述

  • 产品名称Anti-MMP2抗体
    参阅全部 MMP2 一抗
  • 描述
    兔多克隆抗体to MMP2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human MMP2.

    (Peptide available as ab92853.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HT1080; MCF7; PC3; DU145.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab83173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 73 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • 组织特异性Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • 疾病相关Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • 细胞定位Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    Anti-MMP2 antibody 图像

    • All lanes : Anti-MMP2 antibody (ab83173) at 1 µg/ml

      Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 3 : PC3 whole cell lysate (ab3954)
      Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 73 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 44 kDa,52 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 15 minutes

    Anti-MMP2 antibody (ab83173)参考文献

    ab83173 has not yet been referenced specifically in any publications.

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    The ab44848 epitope is at the aminoterminus of the Hemopexin-like domain, and the ab41042 epitope is at the carboxyterminal end of the Hemopexin-like domain. The tw...

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