概述

  • 产品名称Anti-MMP2抗体[2C1]
    参阅全部 MMP2 一抗
  • 描述
    小鼠单克隆抗体[2C1] to MMP2
  • 特异性This antibody is specific for pro and active MMP-2.
  • 经测试应用适用于: ELISA, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to MMP2. Activated recombinant human MMP2

  • 阳性对照
    • Lung, nerve, and various tumors.

性能

应用

Our Abpromise guarantee covers the use of ab7033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
IHC-Fr
IP
WB
  • 应用说明ELISA: Use at a concentration of <0.1 µg/ml. Tested on recombinant protein only.
    IHC-Fr: Use at a concentration of 5 µg/ml.
    IP: Use at an assay dependant dilution.
    WB: Use at a concentration of 2 - 5 µg/ml.

    May be used for western blotting, where a band is seen at 70 (pro form) and 62 kDa (active form) representing the MMP-2 band-pair.

    This antibody does not react with formalin fixed paraffin embedded tissue.
  • 靶标

    • 功能Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • 组织特异性Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • 疾病相关Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • 细胞定位Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 72 kDa gelatinase antibody
      • 72kD type IV collagenase antibody
      • CLG 4 antibody
      • CLG 4A antibody
      • CLG4 antibody
      • CLG4A antibody
      • Collagenase Type 4 alpha antibody
      • Collagenase type IV A antibody
      • Gelatinase A antibody
      • Gelatinase alpha antibody
      • Gelatinase neutrophil antibody
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
      • Matrix Metalloproteinase 2 antibody
      • Matrix metalloproteinase II antibody
      • Matrix metalloproteinase-2 antibody
      • MMP 2 antibody
      • MMP II antibody
      • MMP-2 antibody
      • MMP2 antibody
      • MMP2_HUMAN antibody
      • MONA antibody
      • Neutrophil gelatinase antibody
      • PEX antibody
      • TBE 1 antibody
      • TBE-1 antibody
      see all

    Anti-MMP2 antibody [2C1] (ab7033)参考文献

    This product has been referenced in:
    • Farahani RM  et al. Directed glia-assisted angiogenesis in a mature neurosensory structure: pericytes mediate an adaptive response in human dental pulp that maintains blood-barrier function. J Comp Neurol 520:3803-26 (2012). Read more (PubMed: 22678627) »
    • Ferguson TA & Muir D MMP-2 and MMP-9 increase the neurite-promoting potential of schwann cell basal laminae and are upregulated in degenerated nerve. Mol Cell Neurosci 16:157-67 (2000). Read more (PubMed: 10924258) »

    See all 4 Publications for this product

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    Thank you for contacting us.

    The lot provided is the only current lot available. If they have not yet tried to use this replacement vial, we ask that they please attempt to use this replacement as we feel the issue may be vial specific. Read More

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    I am sorry to hear that these suggestions did not help. When looking at the image provided I noticed that it was very speckled. There are a number of issues which could cause this and many of these may affect antibo...

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    Thank you for contacting us.

    We are sorry to hear of these issues when usingab7033. We recommend using this product at higher concentrations thanpreviouslyused, specifically trying 1:200 and 1:100. We also recommend increasing the concentr...

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    Thank you for contacting us. I am sorry to hear that you are having trouble with our antibody ab7033. While I am in touch with the lab, could you please let me know the following details about your protocol: 1) What antibody amount, i...

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    Thank you for your enquiry. I appreciate your continued patience in this matter. I have received some feedback from some of the sources of the antibodies that you are enquiring about; ab7033 - To follow on with the comments that I have made the...

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    Thank you for your enquiry. Once again I am sorry to hear of the difficulties that you have experienced using these antibodies. I have had a look through the information that you have provided in conjunction with the blot images and I have a few co...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with these antibodies. The results that you have been obtaining are certainly troubling. What concerns me the most are the two bands that you are consistently det...

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    Thank you for your enquiry. That is a very difficult question to answer and is based on the inherent properties of these particular antibodies and the antigen. In this case, you need to use the monoclonal more concentrated, but for another antibody...

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    Thank you for your enquiry. ab16306 can recognize both the pro and active forms of mouse MMP-9. I have updated the datasheet for ab7032 and ab16306. I hope this information helps, please do not hesitate to contact us if you need any more advice ...

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    I believe this is also from your customer who is using ab3159 as well. My recommendations at this point are the same, please see below. If he or she continues to experience difficulty, please let me know and also please include an image of the blots. A...

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