概述

  • 产品名称Anti-MMP19抗体
    参阅全部 MMP19 一抗
  • 描述
    兔多克隆抗体to MMP19
  • 特异性ab53146 detects endogenous levels of total MMP19 protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from human MMP19.

  • 阳性对照
    • WB: Extracts of HuvEc cells IHC-P: Human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab53146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 57 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

靶标

  • 功能Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
  • 组织特异性Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
  • 疾病相关May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Activated by autolytic cleavage after Lys-97.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Matrix metalloproteinase 18 antibody
    • Matrix metalloproteinase 19 antibody
    • Matrix metalloproteinase RASI antibody
    • Matrix metalloproteinase-18 antibody
    • Matrix metalloproteinase-19 antibody
    • MMP 18 antibody
    • MMP 19 antibody
    • MMP-18 antibody
    • MMP-19 antibody
    • MMP18 antibody
    • MMP19 antibody
    • MMP19_HUMAN antibody
    • RASI 1 antibody
    • RASI antibody
    • RASI1 antibody
    see all

Anti-MMP19 antibody 图像

  • All lanes : Anti-MMP19 antibody (ab53146) at 1/500 dilution

    Lane 1 : Extracts of Huv Ec cells with no peptide
    Lane 2 : Extracts of Huv Ec cells with peptide


    Predicted band size : 57 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53146 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

Anti-MMP19 antibody (ab53146)参考文献

This product has been referenced in:
  • Liu RY  et al. Overexpression of asparagine synthetase and matrix metalloproteinase 19 confers cisplatin sensitivity in nasopharyngeal carcinoma cells. Mol Cancer Ther 12:2157-66 (2013). Human . Read more (PubMed: 23956056) »
  • Jackson V  et al. Matrix metalloproteinase 14 and 19 expression is associated with thoracic aortic aneurysms. J Thorac Cardiovasc Surg 144:459-66 (2012). Human . Read more (PubMed: 21955474) »

See all 2 Publications for this product

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