重组
RabMAb

Anti-MMP17抗体[EP1270Y] (ab51075)

概述

  • 产品名称
    Anti-MMP17抗体[EP1270Y]
    参阅全部 MMP17 一抗
  • 描述
    兔单克隆抗体[EP1270Y] to MMP17
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IHC-FoFrmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on human MMP17.

  • 阳性对照
    • Mouse brain lysate, human lymphoma tissue or HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab51075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22022509
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
    • 组织特异性
      Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
    • 序列相似性
      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰
      The precursor is cleaved by a furin endopeptidase.
    • 细胞定位
      Cell membrane. Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Matrix metalloproteinase 17 antibody
      • Matrix metalloproteinase 17 membrane inserted antibody
      • Matrix metalloproteinase-17 antibody
      • Membrane type 4 matrix metalloproteinase antibody
      • Membrane type matrix metalloproteinase 4 antibody
      • Membrane-type matrix metalloproteinase 4 antibody
      • Membrane-type-4 matrix metalloproteinase antibody
      • MMP 17 antibody
      • MMP-17 antibody
      • Mmp17 antibody
      • MMP17_HUMAN antibody
      • MT MMP 4 antibody
      • MT MMP4 antibody
      • MT-MMP 4 antibody
      • MT4 MMP antibody
      • MT4-MMP antibody
      • MT4MMP antibody
      • MTMMP 4 antibody
      • MTMMP4 antibody
      see all

    图片

    • Anti-MMP17 antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 67 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Ab51075 (1:50) staining human MMP17 in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab51075 (1:50).
    • Immunohistochemical analysis of rat basolateral amygdala tissue, staining MMP17 with ab51075.

      Tissue was permeabilized with 0.5% Triton-X 100 before incubating with primary antibody (1/100) for 48 hours at 4°C. An AlexaFluor®568 goat anti-rabbit IgG was used as the secondary antibody.

    文献

    This product has been referenced in:
    • Cepeda MA  et al. Less is more: low expression of MT1-MMP is optimal to promote migration and tumourigenesis of breast cancer cells. Mol Cancer 15:65 (2016). Human . Read more (PubMed: 27756325) »
    • Martín-Alonso M  et al. Deficiency of MMP17/MT4-MMP proteolytic activity predisposes to aortic aneurysm in mice. Circ Res 117:e13-26 (2015). Read more (PubMed: 25963716) »

    See all 6 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (human cell lines)
    Specification
    human cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mrs. Monika Schumacher

    Verified customer

    提交于 Feb 25 2015

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10 %)
    Sample
    Guinea pig Tissue lysate - whole (Liver)
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mrs. Monika Schumacher

    Verified customer

    提交于 Jan 23 2015

    r these details. I am sorry this vial of antibody has not worked for this customer.

    As requested, we are pleased to arrange an alternative free of charge replacement of ab39028. I can confirm that 1 vial of ab39028 has been added to your ord...

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    Thank you for your message and for providing this further information.

    I am sorry there are no further suggestions to help improve the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased t...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will provide us with vital informa...

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    Thank you for your inquiry.

    The concentration of ab51075 is lot dependent. The lot that we have in stock at the moment and of the last vial you receivedhas a concentration of 0.224 mg/mL.

    I also would like to comment on the seemingl...

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    Vielen Dank für Ihren Anruf gestern.

    Ich habe jetzt genauere Information zum Immunogen erhalten:

    Es liegt zwischen den Aminosäuren AA190 und 230 (SwissProt ID Q9ULZ9).

    Ich hoffe, diese Information hilft Ihnen her...

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    Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instr...

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    Thank you for your inquiry. I am happy to confirm that the concentration of lot GR19885 is 0.224 mg/mL. I also would like to comment on the seemingly low concentration: The IgG produced by rabbits have greater affinity and greater sensitivity ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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