Anti-MMP15抗体(ab15475)

概述

  • 产品名称Anti-MMP15抗体
    参阅全部 MMP15 一抗
  • 描述
    兔多克隆抗体to MMP15
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide from the catalytic domain of mouse MMP15 protein.

  • 阳性对照
    • IHC-P: Human placenta tissue. ICC/IF: Jeg3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab15475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 76 kDa.
IHC-P 1/50.

1/50 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.

ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
  • 组织特异性Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI503551 antibody
    • Matrix metallopeptidase 15 membrane inserted antibody
    • Matrix metalloproteinase 15 antibody
    • Matrix metalloproteinase-15 antibody
    • Membrane type 2 matrix metalloproteinase antibody
    • Membrane type matrix metalloproteinase 2 antibody
    • Membrane-type matrix metalloproteinase 2 antibody
    • Membrane-type-2 matrix metalloproteinase antibody
    • MMP 15 antibody
    • MMP-15 antibody
    • MMP15 antibody
    • MMP15_HUMAN antibody
    • MT MMP 2 antibody
    • MT-MMP 2 antibody
    • MT2 MMP antibody
    • MT2-MMP antibody
    • MT2MMP antibody
    • MTMMP 2 antibody
    • MTMMP2 antibody
    • SMCP 2 antibody
    • SMCP-2 antibody
    • SMCP2 antibody
    see all

Anti-MMP15 antibody 图像

  • ICC/IF image of ab15475 stained Jeg3 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15475, 5g/ml) overnight at +4°C. The secondary antibody (green) was ab96899, Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • ab15475 staining MMP15 in human placenta by Immunohistochemistry (FFPE-sections).

Anti-MMP15 antibody (ab15475)参考文献

ab15475 has not yet been referenced specifically in any publications.

Product Wall

Following correspondence with the source of this antibody I have determined that whilst this antibody has not been tested for potential cross reactivity the immunogen sequence shows no homology with other MT-MMPs. I hope this information helps, plea...

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